Radical scavenging capacity and total phenolic content of Ajuga laxmannii (Murray) Benth.
Ajuga laxmannii (Murray) Benth. (Lamiaceae) is an herbaceous plant, distributed in dry regions of Central and Southeast Europe. It’s traditionally used as a galactagogue and for the treatment of inflammation and respiratory infections. The aim of this study was to analyze antioxidant capacity and total phenols and flavonoid contents (TPC and TFC) of 70% ethanol extract of this species. All measurements were performed spectrophotometrically. The antioxidant potential was evaluated using the DPPH and ABTS radical scavenging method. Butylated hydroxyanisole (BHA), α-tocopherol and L-ascorbic acid were used as reference standards. The results obtained by the DPPH test indicated IC50 values of 0.564 mg/ml, while IC50 using the ABTS test was 1.810 mg/ml. They were compared to the control substances which indicate activity of BHA (IC50=0.129; IC50=0.090 mg/ml), α-tocopherol (IC50=0.152; IC50=0.310 mg/ml) and L-ascorbic acid (IC50=0.067; IC50=0.110 mg/ml), respectively. TPC at 101.068 mg GAE/g DW and TFC at 48.946 mg QE/g DW were in correlation with their high antioxidant capacity. These findings indicate that ethanol extract obtained from aerial parts of A. laxmannii possessed significant radical scavenging activity and suggest that it could be recognized as potential source of antioxidant ingredients for the food and pharmaceutical industry.
engleski
2022
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