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Application of rhizobial inoculation in acid soil to obtain high-quality animal feed
Delić, Dušica
Simić, Aleksandar
Buntić, Aneta
Rasulić, Nataša
Kuzmanović, Đorđe
Stajković-Srbinović, Olivera
Abstract: In the field Italian ryegrass (Lolium multiflorum Lam.) was grown in an acid soil with the aim to evaluate the effects of Rhizobium leguminosarum bv. trifolii on its growth. Inoculation with this symbiotic nitrogen-fixing bacteria would have a role in promoting the growth of grass under stress conditions. Italian ryegrass cultivar K-29 was sown on plots of 5 m2 in a randomised complete design with six replications. There were three treatments: inoculation treatment (Rhizobium strain 459), mineral nitrogen fertilizer treatment (50 kg ha-1) and control treatment without inoculation and mineral fertilizer application. Application of mineral N fertilizer in the first harvest had the highest influence on shoot fresh and dry weight, percentage and yield of crude proteins. Plants treated with mineral N achieved the highest fresh and dry weight, 23.53 t ha-1 and 4.21 t ha-1 respectively, and percentage (10.7 %) and yield of crude proteins (445 kg ha-1). Inoculation of ryegrass with Rhizobium significantly increased yield and plant quality in the first harvest compared to control. In inoculation treatment, shoot fresh weight (17.43 t ha-1) was 50% higher than in control treatment (11.4 t ha-1). Shoot dry weight and crude protein yield (2.99 t ha-1 and 333 kg ha-1) were higher by 25% and 64%, compared to control. In the second harvest, there was no significant influence of inoculation and mineral N on yield parameters. Although mineral N application in Italian ryegrass growing in the acid soil was the most efficient, Rhizobium inoculation also significantly increased plant growth and yield quality compared to control. Results indicated that Rhizobium inoculation can reduce mineral N application in Italian ryegrass cultivation which can be used as cost-effective way of its production in the soils with limited fertility.
engleski
2017
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Lolium multiflorum, inoculation, Rhizobium, yield, crude proteins.