Naslov (srp)

The effect of pre-inoculation on wheat seed protection

Autor

Pavlović, Jelena
Knežević, Magdalena
Milinković, Mira
Maksimović, Jelena
Jovković, Marina
Đikanović, Nikola
Buntić, Aneta

Opis (eng)

Abstract: Pre-inoculation of seeds allows farmers to avoid daily inoculation, to focus on sowing and protecting seeds in the earliest period of seed germination. By using pre-inoculated seeds, farmers save time during sowing and reduce manpower requirements. Pre-inoculated seeds maintain bacterial viability for as long as possible, and this depends on a number of factors. The long-term viability of bacterial cells on pre-inoculated seeds depends on the bacterial species and the ability of the bacteria to survive in specific conditions, as well as the adhesive substances and cell protectors in the inoculant formulation. Inoculation is more common with liquid fertilizers than with inoculants that act as biopesticides. In this study, the bacterial strain Priestia megaterium BHC 5.5 was used for pre-inoculation of wheat seeds and protection of seedlings against Fusarium poae. In our previous research, the bacterial strain BHC 5.5 showed the ability to suppress the growth of the phytopathogenic fungus F. poae. Two hundred wheat seeds were soaked in a bacterial suspension (50 mL, 109 CFU mL⁻¹) for 5 minutes and then allowed to air dry. The dried seeds were stored at 22°C for periods of 0, 1, 2 and 3 months. Pre-inoculated seeds (20 seeds per treatment) without and with F. poae infection were placed monthly in Petri dishes on moist filter paper. Infection of pre-inoculated seeds was performed by placing the seeds in a Petri dish with F. poae for 10 minutes. In addition, the germination of uninoculated and infected uninoculated seeds was done in same manner. Petri dishes were kept for one week in a transparent sealed box. Final seed germination percentage (FG %) was calculated: germinated seeds in samples / total planted seeds x 100. The relative seed germination index (RSGI%) was calculated as follows: germinated uninfected pre-inoculated seeds/ germinated infected preinoculated seeds x 100. In each month, the pre-inoculated wheat seeds showed FG of 100%, except for the germination of seeds stored for two months (90%).The final seed germination percentage of the infected preinoculated seeds were 90%, 85%, 70% and 55% of the seeds stored for 0, 1, 2 and 3 months, respectively. The RSGI of the infected pre-inoculated seeds was the same as the FG for seeds stored for 0, 1 and 3 months. For the pre-inoculated seeds for 2 months, the RSGI was 77%. The FG of the infected seeds was only 40%. Pre-inoculation of wheat seeds with the bacterial strain P. megaterium BHC 5.5 provided protection to the seeds and increased germination from 40% to 85% in the treatment where the pre-inoculated seeds were stored for one month. Pre-inoculation two months before sowing also showed satisfactory results in wheat seed protection. Further research will include the development of inoculant formulation with BHC 5.5 as a biocontrol agent in the protection of pre-inoculated wheat seeds.

Jezik

engleski

Datum

2025

Licenca

© All rights reserved

Predmet

pre-inoculation, wheat seed, Priestia megaterium, seed germination, F. poae biocontrol, seed protection

Deo kolekcije (1)

o:508

Institut za zemljište