Naslov (eng)

Application of Bacillus spp. in plant protection and growth promotion of cereals

Autor

Jovković, Marina
Knežević, Magdalena
Dervišević, Marina
Maksimović, Jelena
Jevđenović, Galina
Milinković, Mira
Buntić, Aneta

Opis (eng)

Abstract: The research objective is the application of new bacterial isolates as an alternative to chemical pesticides for controlling plant diseases caused by Fusarium spp., aiming to reduce reliance on conventional interventions, minimize environmental impacts, thus fostering a shift towards environmentally friendly plant disease management strategies. In this study, the effects of four Bacillus spp. (BHC 2.3, BHC 2.4, BHC 4.5, BHC 4.7) isolated from two soil samples on suppressing the Fusarium spp. plant pathogens (F. poae, F. proliferatum, F. oxysporum and F. graminearum), as well as their effect on seed germination (wheat, oat and barley) during the fungal infection were determined. Basic chemical analyses of soil samples used for bacterial isolation and plant growth promoting experiments were performed. Antifungal activity of isolates was tested in vitro on PDA medium, seed germination test was performed in Petri dishes on filter paper, while the ability of isolates to promote plant growth under semi-controlled conditions was also tested. Inhibition percentage of mycelial growth of F. poae was up to 20% (for isolate BHC 4.5) and up to 15% (for isolate BHC 4.7). Seed germination test indicated the effectiveness of seed inoculation by selected bacterial isolates. In the case of infected seeds, germination percentage was up to 80% (wheat) and up to 90% (barley and oat), without bacterial inoculation. On the other hand, germination percentage for infected seeds inoculated by all four applied isolates was increased. Results of the experiment performed under semi-controlled conditions showed that bacterial isolate BHC 4.5 had the best overall effect on the shoot length of all used cereals. The results of this study indicate the potential of different Bacillus spp. soil isolates for formulating new biofertilizers to be used in plant protection and plant growth promotion of cereals.

Jezik

engleski

Datum

2024

Licenca

© All rights reserved

Predmet

Bacillus spp., PGP bacteria, seed germination, oat, barley and wheat seedling shoots

Deo kolekcije (1)

o:508

Institut za zemljište