Naslov (srp)

Bacterial communities in acidic soil

Autor

Jovičić-Petrović, Jelena
Milinković, Mira
Karličić, Vera
Lalević, Blažo
Kljujev, Igor
Raičević, Vera

Opis (eng)

Abstract: Acidification is one of the main types of soil degradation in Serbia, as a result of excessive use of mineral fertilization, pollution, as well as reduction of soil organic matter. Increased soil acidity directly affects plant nutrition and food productivity, at the same time leading to biodiversity changes. Bacterial diversity in soil is recognized as the main pillar of soil quality, ecosystem stability, climate change resilience, and represents an important element of sustainable agriculture. The diversity and abundance of bacteria in soil are strongly related to various abiotic factors, particularly to soil pH as one of the major determinants shaping their community structure. The main objective of the research was to access the bacterial community in agricultural acid soils using metagenomic approach. Soil samples were taken at three locations: cornfield near Zaječar (Eastern Serbia), apple and raspberry orchards near Čačak (Central Serbia). The representative samples were subjected to sequencing of V3 and V4 regions of 16S rRNA gene using Illumina® MiSeq™. Besides microbiome, physico-chemical analyses were performed, including mechanical composition, adsorptive complex properties, and basic parameters of soil fertility. Soil samples from Čačak have strongly acidic reaction, belonging to class of clay loam with a significant share of powder fraction. Soil from Zaječar is heavy clay with 48.80% of the clay fraction, and middle acidic reaction. Cation exchange capacity (CEC) is the middle level, and saturation of the adsorptive complex with base cations is weak in raspberry orchard soil, while soil under apple and corn showed high levels of CEC and middle saturation of adsorptive complex with base cations. Soil from apple orchard is characterized by low humus content and low content of available phosphorous (P), and middle P content in the raspberry orchard and cornfield. Available K content was similar for three analyzed fields (35.60-37.90 mg 100g-1). In all of the studied soils, the most abundant phyla were Firmicutes, Proteobacteria, and Actinobacteria (each above 20%), which are the usual predominant phyla in the fertile soil. Proteobacteria composition showed differences between the soil samples, with higher share of Enterobacteriales in cornfield soil. Acid soil from Zaječar had 4.89% abundance of Chloroflexi, while the soils from Čačak included six additional phyla besides Chloroflexi (with more than 1% abundance), indicating significantly higher biodiversity. After the three most common phyla, Acidobacteria were predominantly abundant, and the presence of these oligotrophic taxa is characteristic of less fertile soil. Actinobacteria are mainly related to neutral or alkaline soil, but in recent decade acidotolerant Actinobacteria are being highlighted in terms of maintaining ecosystem balance, and raise of pH.

Jezik

engleski

Datum

2024-11-29T09:48:25.634Z

Licenca

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Institut za zemljište