Naslov (eng)

Wound healing potential of black raspberry seed oil

Autor

Krgović, Nemanja
Stojković, Dejan
Dimitrijević, Milena
Menković, Nebojša
Šavikin, Katarina
Živković, Jelena

Opis (eng)

The skin is subject to various influences from the external environment that lead to minor or serious problems and diseases. Among the most prevalent is trauma, an injury characterized by disrupting the normal anatomical structure and function of the skin caused by a cut, a blow or a burn, which consequently makes the body become more susceptible to infection. On the basis of numerous animal experiments and human studies, plant oils are recognized to play an important role in regenerative processes. This study aimed to investigate the wound-healing potential of black raspberry (Rubus occidentalis L., Rosaceae) seed oil, as well as its chemical characterization and antimicrobial activity against the most common skin pathogens. GC-MS analysis was performed to identify the chemical composition of fatty acids in the black raspberry seed oil and revealed that polyunsaturated fatty acids (PUFA) dominated – the most abundant were linoleic (C18:2n-6; 39.30%) and α-linolenic (C18:3n-3; 30.49%) acids, followed by monounsaturated oleic acid (C18:1n-9; 18.94%). The black raspberry seed oil demonstrated significant antimicrobial activity against the bacterial strains Salmonella Typhimurium, Escherichia coli and Bacillus cereus, in addition to antifungal activity against micromycetes Aspergillus fumigatus, A. niger, A. versicolor and Trichoderma viride. The wound healing activity, in terms of migratory ability, was evaluated by scratch assay in the same cell line, whereby cell migration into the wounded area was monitored using an Inverted Microscope. The closure of the gap distance (wound closure) was found to be approximately 2-fold higher in cells treated with 200 µg/mL of black raspberry seed oil (41.77%) than in non-treated cells (17.34%). The results showed that black raspberry seed oil is a rich source of PUFA, with promising potential in the treatment of skin wounds associated with infections.

Jezik

engleski

Datum

2024

Licenca

© All rights reserved

Predmet

Rubus occidentalis, seed, oil, fatty acids, skin wounds

Deo kolekcije (1)

o:2594

Radovi nastavnika i saradnika Instituta za proučavanje lekovitog bilja "Dr Josif Pančić"