Naslov (eng)

Influence of different solvents on antioxidant capacity of extrcts from red elderberry (Sambucus racemosa L.) fruit.

Autor

Miljković, Vojkan
Đorđević, Biljana
Todorović, Zoran
Veljković, Vlada
Marković, Marija
Nikolić, Ljubiša
Nikolić, Goran

Publisher

Opis (eng)

Elderberries (family Adoxaceae, genus Sambucus) consist of 9 species. Elderbrrry fruit is rich in phytochemicals, of which the phenolic compounds are the most common. Phenols are known for their antioxidant properties. Deep eutectic solvents (DES) represent a new class of solvents and are considered as ideal substitutes of conventional organic solvents. These solvents provide high efficiency and offer a high yield of a great variety of compounds. The subject of this study was determination of antioxidant capacity of extract from S. racemosa L. (red elderberry) provided with different solvents. Dried and grinded (0.2/6 mm) fruit of red elderberry (1 g) was extracted with 10 cm of the corresponding solvent (acetone, methanol, chlorophorm, choline chloride, glycerol (ChCl:G) or choline chloride urea (ChCl:U)). The extraction was carried during 180 minutes at 60 C in a thermostated chamber with constant stirring. Antioxidant acitivity of extracts was determined spectrophotometrycally by using DPPH assay. The extraction yields obtained by ChCl:U (1:2), acetone, chlorophorm, methanol and ChCl:G (1:2) where 6%, 6.5%, 0.6%, 15.2%, and 18% respectively. IC50 value of extracts obtained by diiferent solvents are decreasing in the folloving order: chlorophorm 2,940 mg/cm3, acetone 1,391 mg/cm3, methanol 0,217 mg/cm3, ChCl:G 0,138 mg/cm3, ChCl:U 0,058 mg/cm3. Results in this study shous that extraxt obtained with ChCl:U posses best antioxidant activity. Based on the results obtained in this study, it is concluded that DES present better solvents for the extraction of extractive substances from red elderberry fruit than conventional solvents which are often in use. Extraction yield obtained with ChCl:G is higher than ones with ChCl:U, acetone, chlorophorm or methanol. Also, DES used as solvents for extraction provides extracts with possesses better antioxidant properties than conventional solvents.

Jezik

engleski

Datum

2019

Licenca

© All rights reserved

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Radovi Instituta za šumarstvo